All ties are set to take place on Saturday 31 October 2026.
Winning clubs at this stage will receive £900 from the competition prize fund, while losing teams will collect £275.
Maghull v Retford
Chadderton v Bottesford Town
Thornton Cleveleys v Billingham Town
Holker Old Boys v Winsford United
Shildon v Carlisle City
West Didsbury & Chorlton v Prestwich Heys
Bishop Auckland v Atherton LR
Cheadle Town v Droylsden
FC St Helens v Boldon CA
North Shields v Brigg Town CIC
Woolton v Durham United
Northwich Victoria v Yarm & Eaglescliffe
Sheffield v South Liverpool
Nelson v Burscough
Bilston Town v Heanor Town
Birstall United v Cinderford Town
Ashby Ivanhoe v Gornal Athletic
Abbey Hulton United v Brocton
AFC Bridgnorth v Desborough Town
Atherstone Town v GNG Oadby Town
Lye Town v Eastwood Community
Cirencester Town v Longlevens
Southwell City v Rugby Town
Gresley Rovers v Ludlow Town
Sherwood Colliery v Heather St Johns
Staveley MW v Hinckley
Harrowby United v Sutton United (Birmingham)
Belper United v Northampton On Chenecks
Kings Langley v Holland
Halesworth Town v Hadleigh United
Ampthill Town v Haverhill Rovers
Belstone v Wisbech Town
Heybridge Swifts v Everett Rovers
Risborough Rangers v Great Yarmouth Town
Cockfosters v Norwich United
Lymore Gardens v Biggleswade United
Tring Athletic v Hertford Heath
Harpenden Town v West Essex
St Neots Town v Saffron Walden Town
Colney Heath v Benfleet
White Ensign v Wormley Rovers
Lingfield v Crawley Down Gatwick
Hassocks v Eastbourne United
Rusthall v Haywards Heath Town
Larkfield & New Hythe v East Grinstead Town
Harefield United v Snodland Town
Fisher v AFC Varndeanians
Northwood v Reading City
Pagham v AFC Greenwich Borough or Tooting Bec
Tunbridge Wells v Ash United
SE Dons v Hythe Town
Cove v Lancing
Phoenix Sports v Faversham Strike Force
Andover New Street v Mangotsfield United
Downton v New Milton Town
Cowes Sports v Buckland Athletic
Hallen v Hythe & Dibden
Bovey Tracey v Brixham
Wantage Town v St Blazey
Honiton Town v Devizes Town
Shepton Mallet v Bridgwater United
Amesbury Town v Highworth Town
Portishead Town v Clevedon Town
Elburton Villa v Sturminster Newton United